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| Product Name | Ral GDS Rap-binding domain (Ral GDS RBD) (Agarose) |
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| Description | Purity ~90%. Purified by glutathione-agarose chromatography. A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), expressed in E. coli. Provided bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rap1 (Mr 22kD), not GDP-Rap1 from cell lysates. Recommended for use with cells that have high levels of Rap1 expression (e.g., platelets). Applications: Suitable for use in Affinity Precipitation Assay, Affinity Precipitation and Immunoblot Analysis. Affinity Precipitation Assay: 30ug (~47ul of slurry) precipitated GTP-Rap1 from HEK293 lysates under the conditions described in the affinity precipitation protocol. The precipitated GTP-Rap1 was detected by Western Blot analysis using 2ug/ml of polyclonal anti-Rap1. The antibody detected two additional unknown proteins of Mr ~62 and 88kD. Affinity Precipitation and Western Blot Analysis: Samples were treated as described for affinity precipitation assay: HEK293 lysate i |
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| Size | 650ug |
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| Concentration | n/a |
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| Applications | WB |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NM_018975.2, SwissProt: Q9NYB0 |
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| Catalog # | R1035-50 |
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| Order / More Info | Ral GDS Rap-binding domain (Ral GDS RBD) (Agarose) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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