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| Product Name | Procathepsin B, Recombinant, Human, aa18-339, His-Tag (CTSB, CPSB) |
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| Description | Purity ~95% (SDS-PAGE). Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28kD) by adding an equal volume of 0.2M NaOAc, 5mM EDTA pH 4.0 containing fresh 5mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3M excess over MMTS) to the enzyme solution. Source: Recombinant protein corresponding to aa18-339 of human Procathepsin B, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~38.2kD Biological Activity: >5000mu/mg (1U=1umole/min, after |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | CTSB, Gene ID: 1508, SwissProt: P07858 |
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| Catalog # | 301270 |
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| Order / More Info | Procathepsin B, Recombinant, Human, aa18-339, His-Tag (CTSB, CPSB) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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