| 产品详情 |
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| Product Name | Cfr13I (AsuI) |
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| Description | Sequence: 5'-G^G N C C-3' 3'-C C N G^G-5' Restriction Enzyme Buffer A, 10X (R1625): Supplied to simplify buffer selection for double digests. Enzyme Properties: Number of Recognition Sites in DNA: Lambda: 74 PhiX174: 2 pBR322: 15 pUC57: 8 pUC18/19: 6 pTZ19R/U: 6 M13mp18/19: 4 Methylation Effects: Cfr13I does not cut GGNCm5C. Blocked by overlapping Dcm and CG methylation. Stability during Prolonged Incubation: A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Compatible Ends: CpoI, Eco47I, Psp5II, SanDI: G^G(A/T)CC Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Cfr13I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Cfr13I, more than 95% of the DNA fragments can be ligated at a 5'-termini concen |
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| Size | 1000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C3300 |
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| Price | |
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| Order / More Info | Cfr13I (AsuI) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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