产品名称 Cfr10I (BsrFI) from UNITED STATES BIOLOGICAL
产品货号 C3275
产品价格 现货询价,电话:010-67529703
产品规格 200U
产品品牌 UNITED STATES BIOLOGICAL
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Product NameCfr10I (BsrFI)
DescriptionSequence: 5'- R ^C C G G Y -3' 3'- Y G G C C^ R -5' Source: E.coli that carries the cloned cfr10IR gene from Citrobacter freundii RFL10 Concentration: 10 units/ul Unit Definition: One unit is defined as the amount of Cfr10I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Incubation Temperature: 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM potassium phosphate (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-45: Restriction Enzyme Buffer for Cfr10I, 10X: Supplied as a liquid in 100mM Tris-HCl, pH7.5, 50mM magnesium chloride, 1M sodium chloride, 10mg/ml BSA, 0.2% Triton X-100. R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 330mM Tris-acetate, pH 7.9, 100mM magnesium acetate, 660mM potassium acetate, 1mg/ml BSA. Enzyme Properties: Star Activity: An excess of Cfr10I (10u/ug DNA x
Size200U
Concentrationn/a
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Catalog #C3275
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