产品名称 MssI (PmeI) from UNITED STATES BIOLOGICAL
产品货号 M4692-60
产品价格 现货询价,电话:010-67529703
产品规格 250U, 1250U
产品品牌 UNITED STATES BIOLOGICAL
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Product NameMssI (PmeI)
Description5'-G T T T^A A A C-3' 3'-C A A A^T T T G-5' Concentration: 5u/ul Source: Methylobacterium species Dd 5-732 Unit Definition: One unit is defined as the amount of Mssl required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Diluent Buffer : 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04 Restriction Enzyme Buffer E, 10X: Dilute to 1X for 100% Mssl digestion. 10mM Tris-HCl, pH 7.5, 10mM MgCl2 and 0.1mg/ml BSA. Incubation Temperature: 37°C
Size250U, 1250U
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Catalog #M4692-60
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