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| Product Name | DOT1L, Chemiluminescent, BioAssay™ Kit (KMT4, KIAA1814, Histone Methyltransferase DOT1L) |
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| Description | The DOT1L Chemiluminescent BioAssay™ Kit is designed to measure DOT1L activity for screening and profiling applications. Test Principle: The key to the DOT1L Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Applications: Great for studying enzyme kinetics and HTS applications. Kit Components: Strip plate, 1x96 wells DOT1L, 1x50ug 100uM S-adenosylmethionine, 1x250ul DOT1L substrate, 1x250ul Primary Antibody 25, 1x12.5ul Secondary HRP-labeled Antibody 2, 1x10ul 4x HM |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 298405 |
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| Order / More Info | DOT1L, Chemiluminescent, BioAssay™ Kit (KMT4, KIAA1814, Histone Methyltransferase DOT1L) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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