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| Product Name | COT, Recombinant, Human, GST-Tag |
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| Description | Purity ~75%. Human Cot/Tpl2 (GenBank Accession No. NM_005204), (aa30-397), with N-terminal GST-HIS6-tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: ~40pmol/min/ug Assay Conditions: 100ul of 240nm biotinylated MEK1 peptide was coated onto a neutravidin plate for 1 hour at 37°C. After overnight blocking with TBS superblock, the reaction was done with 10uM ATP for 60 minutes at RT, and phospho-MEK1 was detected using an anti-phospho-MEK1 antibody. Specific activity was determined relatively by comparing with a standard kinase under the same condition. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | COT, Recombinant, Human, GST-Tag (Cancer Osaka Thyroid Oncogene, c-COT, EST, ESTF, Mitogen-activated Protein Kinase Kinase Kinase 8, MAP3K8, MEKK8, Proto-oncogene c-Cot, Serine/threonine-protein Kinase Cot, Tumor Progression Locus 2, TPL2, TPL-2) |
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| Gene, Accession, CAS # | Accession: NM_005204 |
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| Catalog # | C7904-43 |
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| Price | |
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| Order / More Info | COT, Recombinant, Human, GST-Tag from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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