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| Product Name | Monosodium urate (MSU), ready-to-use |
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| Description | NLRP3/NALP3 inflammasome activator. Specially crystallized and tested for biological activity. Stimulates the caspase-1 activating NLRP3/NALP3 inflammasome, resulting in the production of active IL-1beta and IL-18. Activation of THP-1 cells by uric acid: 1. THP-1 cells are cultured in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum (FCS), 50uM β-mercaptoethanol, 2mM L-glutamine and penicillin/streptomycin. THP-1 cells are grown in suspension to a density of 1.5x10e6 cells/ml in tissue culture flasks. 2. Treat THP-1 cells for 3 hours with 0.5uM PMA the day before stimulation. 3. Wash cells with PBS and plate them in a 12-well plate at a density of 6x10e5 cells/well in RPMI medium. 4. The next day, wash cells with PBS and add 500ul Optimem (Invitrogen). 5. Stimulate the cells for 6 hours by addition of 50-300ug/ml of the suspension of uric acid (vortex the suspension just before use). 6. As a positive control, 5mM of ATP is incubated for 6 hours. Detection of the |
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| Size | 10mg |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 45039 |
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| Order / More Info | Monosodium urate (MSU), ready-to-use from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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