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| Product Name | Mesenchymal Stem Cells, Mouse, Freezing Medium |
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| Description | Description: Mouse MSC freezing medium was optimized for cryopreservation of our Mouse mesenchymal stem cells (mMSC). This product contains fetal bovine serum. Each lot is tested for sterility. Procedure for Freezing mMSC: 1. Freeze mouse MSC cells when they reach > 95% confluence. 2. Warm Dulbecco's PBS (without Ca2+ and Mg2+ bath. Thaw mMSC freezing medium at room temperature.) and 0.05% trypspin/EDTA in a 37°C water 3. Remove mMSC growth medium, rinse mMSC with Dulbecco's PBS, and incubate the cells with trypsin/EDTA solution until approximately 90% of the cells begin to detach. Monitor the cells under a microscope to avoid over-trypsinization. 4. Add fetal bovine serum equal to 1/10 volume of the trypsin/EDTA solution to neutralize trypsin. Gently shake the culture vessel to mix. 5. Transfer the cell suspension into a 15ml conical tube. Take a small aliquot of the suspension to count cells. Centrifuge the cell suspension 250 x g for 5 minutes at room temperature. 6. Carefully remov |
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| Size | 50ml |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | M3005-34C |
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| Order / More Info | Mesenchymal Stem Cells, Mouse, Freezing Medium from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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