| 产品详情 |
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| Product Name | Replication-associated Protein, Recombinant, African Cassava Mosaic Virus, aa1-358, His-Tag (AC1) |
| Description | Purity ~90% (SDS-PAGE). Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all giniviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. Source: Recombinant protein corresponding to aa1-358 from african cassava mosaic virus |
| Size | 10ug, 50ug, 100ug |
| Concentration | n/a |
| Applications | n/a |
| Other Names | 40.4kD protein, Protein AC1, Protein AL1 |
| Gene, Accession, CAS # | SwissProt: P14982 |
| Catalog # | 375040 |
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| Order / More Info | Replication-associated Protein, Recombinant, African Cassava Mosaic Virus, aa1-358, His-Tag (AC1) from UNITED STATES BIOLOGICAL |
| Product Specific References | n/a |
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