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| Product Name | PKA Catalytic Subunit, Bovine Heart |
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| Description | Purity ~95% (SDS-PAGE). Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts. Specific Activity: ~20,000units/ug protein Unit Definition: One unit is defined as the amount of enzyme required to incorporate 1pmol phosphate into Kemptide substrate per min at 30°C. Assay Conditions: The activity of the PKA catalytic subunit is assayed in 40mM Tris-HCl, 20mM magnesium acetate, 200uM [gamma-32P]ATP (500-1000cpm/pmol) with 130uM Kemptide substrate in a total volume of 60ul. The reaction is incubated at 30°C for 5min and terminated by sp |
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| Size | 25ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | PKA Catalytic Subunit, Bovine Heart (cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 217670 |
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| Order / More Info | PKA Catalytic Subunit, Bovine Heart from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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