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| Product Name | Tissue cDNA, First Strand, Human Adult Normal, Lung, Trachea, BioGenomics™ |
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| Description | BioGenomics™ Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection. Description Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65°C for 10 minutes. The cDNA is in 1X RT buffer. (1X RT Buffer: 50mM Tris-CI, pH 8.3, 75mM KCI, 3mM MgCI2, 10mM DTT). The estimated cDNA concentration is about 2.5ng/ul. 1ul cDNA is sufficient for one PCR reaction. Quality Control 1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of |
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| Size | 10 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | T5595-0155 |
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| Order / More Info | Tissue cDNA, First Strand, Human Adult Normal, Lung, Trachea, BioGenomics™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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