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| Product Name | F8 (Coagulation Factor VIII) BioAssay™ ELISA Kit (Human) |
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| Description | The F8 (Coagulation Factor VIII) BioAssay™ ELISA Kit (Human) is a quantitative sandwich assay for the detection of F8 in human plasma. Detection Range: 0.313-20ng/ml Sensitivity: <0.188ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F8. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to F8. Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F8, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F8 in the sample is then determined by |
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| Size | 48 tests, 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | SwissProt: P00451-2 |
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| Catalog # | 355545 |
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| Price | |
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| Order / More Info | F8 (Coagulation Factor VIII) BioAssay™ ELISA Kit (Human) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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