产品名称 TaaI (Tsp4CI) from UNITED STATES BIOLOGICAL
产品货号 T0290
产品价格 现货询价,电话:010-67529703
产品规格 200U, 1000U
产品品牌 UNITED STATES BIOLOGICAL
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Product NameTaaI (Tsp4CI)
Description5'-A C N^G T-3' 3'-T G^N C A-5' Unit Definition: One unit is defined as the amount of Taal required to digest 1ug of lambda DNA in 1 hour at 65°C in 50ul of reaction buffer. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Storage Buffer: 10mM Tris-HCl, pH 7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Methylation Effects: Dam: never overlaps - no effect Dcm: never overlaps - no effect CpG: may overlap - cleavage impaired EcoKl: may overlap - effect not determined EcoBl: may overlap - effect not determined Number of Recognition Sites in DNA: Lambda: 187, PhiX174: 15 pBR322: 14, pUC57: 8, pUC18/19: 8, pTZ19R/U: 7, M13mp18/19: 31 Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of
Size200U, 1000U
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Catalog #T0290
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