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| Product Name | Interleukin 1 alpha, Human, BioAssay™ ELISpot Kit |
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| Description | Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-1α. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-1alpha binds to antibody coated microtiter plate,coated with human IL-1alpha capture antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbound proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-1-alpha. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-1alpha secreting location. Each individual spot representing an individual human IL-1alpha secreting cell. Background: IL-1α is a member of interleukin 1 family. IL-1α and IL-1β recognize the same IL-1 receptor and share a number of similar biological |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | Interleukin 1 alpha, Human, BioAssay™ ELISpot Kit (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | I7663-02J |
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| Order / More Info | Interleukin 1 alpha, Human, BioAssay™ ELISpot Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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