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| Product Name | Cathepsin K Activity, BioAssay™ Kit |
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| Description | Contains complete reagents for measuring cathepsin K activity by fluorometer or fluorescence plate reader. Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-K Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-K substrate sequence LR labeled with AFC amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The cathepsin-K assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2097-69B |
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| Order / More Info | Cathepsin K Activity, BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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