| Description | Cathepsin K (CTSK, EC 3.4.22.38), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin. Intended Use: High-throughput adaptable assay to screen, study and characterize the potential inhibitors of Cathepsin K. Test Principle: The Cathepsin K Inhibitor Screening BioAssay™ Kit utilizes the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. Kit Components: CTSK Reaction Buffer, 1x15ml ?CTSK Reagent, 1x100ul?Cathepsin K (Human), 1x10ul ?CTSK Substrate, Ac-LR-AFC, 10mM, 1x200ul ?CTSK Inhibitor, FF-FMK, 1mM, 1x20ul |
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