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| Product Name | LSD1, Fluorescent, BioAssay™ Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110) |
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| Description | LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development. Intended Use: The LSD1 BioAssay™ Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1. Test Principle: The key to the LSD1 BioAssay™ Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (lambda ex= 530±13nm, lambda em= 590±18nm) is measured using a plate reader. Contraind |
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| Size | 384 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | SwissProt: O60341 |
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| Catalog # | 298428 |
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| Order / More Info | LSD1, Fluorescent, BioAssay™ Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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