| 产品详情 |
| Edit |   |
|---|
| Product Name | A431 Calyculin A Control Lysate |
|---|
| Description | Calyculin A is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Human carcinoma A431 cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phospho-Akt (Thr-34), as compared to untreated, control cell lysates. Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with Calyculin A at a final concentration of 10nM for 30 minutes at 37°C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer. Applications: SDS-PAGE/Western blotting 20ml/well End user should determine optimal quantity for their particular applications and experiments. |
|---|
| Size | 100ug |
|---|
| Concentration | n/a |
|---|
| Applications | WB |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | A0001-52 |
|---|
| Price | |
|---|
| Order / More Info | A431 Calyculin A Control Lysate from UNITED STATES BIOLOGICAL |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|