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| Product Name | Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF, Urogastrone) |
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| Description | Purity ≥ 95% by SDS-PAGE. Purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli. AA Sequence: MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR Biological activity: The ED50 for this effect is < 0.89 ng/ml. Measured in a cell proliferation assay using mouse Balb3T3 cell. Activity Assay: 1. Cell line: Balb-3T3 (Mus musculus (mouse)) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium - serum free RPMI 1 |
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| Size | 100ug, 500ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NP_001954 |
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| Catalog # | E3374-03D |
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| Order / More Info | Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF, Urogastrone) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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