产品名称 ADP/ATP Ratio Assay Kit, BioAssay™ from UNITED STATES BIOLOGICAL
产品货号 A0901-76A
产品价格 现货询价,电话:010-67529703
产品规格 96 tests
产品品牌 UNITED STATES BIOLOGICAL
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Product NameADP/ATP Ratio Assay Kit, BioAssay™
DescriptionChanges in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis. ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. Luciferase ATP+D-luciferin+O2 oxyluciferin+AMP+PPi+CO2+light In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with
Size96 tests
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Catalog #A0901-76A
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Order / More InfoADP/ATP Ratio Assay Kit, BioAssay™ from UNITED STATES BIOLOGICAL
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