| 产品详情 |
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| Product Name | Rsk1, active, Recombinant, Rat |
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| Description | Purity ~95%. Purified using Ni2+/NTA-agarose. Activated with p42 MAPK and PDK1, and repurified by glutathione-, heparin-, and Ni2+/NTA-agarose chromatographies. Recombinant full-length rat RSK1 (residues 1-end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags. Expressed in baculovirus in Sf21 insect cells Specific Activity: ~4000-5000U/mg, where one unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM Rsktide per minute at 30°C with a final ATP concentration of 100uM. Quality Control Testing: Kinase Assay: 0.3-3.8ng phosphorylates 30uM RSKtide. Assay background was subtracted from the actual counts to yield the results shown below. MS Tryptic Fingerprint: Confirmed product identity as rat RSK1 with the translated sequence. SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of RSK1 active. |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Rsk1, active, Recombinant, Rat (Ribosomal S6 Kinase 1, RSK-1, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP kinase 1a, Mapkapk1a, MAPKAPK-1a, 90kD Rbosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, Ribosomal Protein S6 Kinase alpha-1, Rps6ka1, S6K-alpha-1) |
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| Gene, Accession, CAS # | SwissProt: Q15418 |
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| Catalog # | R9399-08A |
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| Order / More Info | Rsk1, active, Recombinant, Rat from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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