| 产品详情 |
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| Product Name | ImmunoTek™ TMB |
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| Description | ImmunoTek™ TMB (3,3',5,5'-Tetramethylbenzidine) Stable Liquid Substrate for HRPO, 1.25mM, Soluble Tetramethylbenzidine (TMB) is an excellent substrate for detecting horseradish peroxidase (HRP) labeled probes. TMB produces a soluble end product which is blue in color that is quantitated at 650nm, providing an ultrasensitive quantitative substrate system. TMB has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color wth an adsorption maximum at 653nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 5.9x10e4 mol-1 cm-1) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMB is a single component reagent stable at room |
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| Size | 250ml |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | ImmunoTek™ TMB (3,3',5,5'-Tetramethylbenzidine) Stable Liquid Substrate for HRPO, 1.25mM, Soluble |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | T6005 |
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| Order / More Info | ImmunoTek™ TMB from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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