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| Product Name | ImmunoTek™ TMB Stable Liquid Substrate 1.56mM for HRPO (3,3',5,5'-Tetramethylbenzidine) |
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| Description | ImmunoTek™ TMB Stable Liquid Substrate 1.56mM for HRPO (3,3',5,5'-Tetramethylbenzidine) TMB produces a soluble end product which is blue in color that is quantitated at 650nm. Upon addition of an equal volume of 0.5M/Liter HCl, the resulting yellow chromogen is quantitated at 450nm. The yellow product is stable at room temperature for at least 1 hour displaying less than a 1% loss in absorbance during this time interval. 1.56mM TMB is recommended for manual assays. Benzidine and its derivatives are well documented electron donors for the horseradish peroxidase (HRP) /H2O2 oxidation/reduction system. These amines are known to be potent carcinogens. The synthesis of TMB by Holland, et al., provided a reagent more sensitive than benzidine and much less hazardous due to ortho methylation. Using H2O2 as substrate, the reaction of TMB with HRP proceeds through 2 phases. First, a charge transfer complex, the result of a 1-electron oxidation is formed. This is a blue product displaying an abso |
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| Size | 250ml |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | 3,3',5,5'-Tetramethylbenzidine |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | T6010 |
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| Order / More Info | ImmunoTek™ TMB Stable Liquid Substrate 1.56mM for HRPO (3,3',5,5'-Tetramethylbenzidine) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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