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| Product Name | Caspase 3 BioAssay™ ELISA Kit (CPP-32, Apoptain, Yama, SCA-1) |
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| Description | This Caspase 3 ELISA Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex. = 400nm and Em. = 505nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected. Kit Components: C2087-12A: Cell Lysis Buffer 1x25ml * C2087-12B: Coating Buffer 1x10ml * C2087-12C: Antibody, 20x 1x500ul C2087-12D: Blocking Buffer 1x15ml * C2087-12E: Incubation Buffer 1x100ml * C2087-12F: DTT (1M) 1x400ul C2087-12G: DEVD-AFC Substrate 1x500ul C2087-12H: Positive Control 1x10units C2087-12J: Microtiter Plate 1x12x8wells |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-12 |
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| Order / More Info | Caspase 3 BioAssay™ ELISA Kit (CPP-32, Apoptain, Yama, SCA-1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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