| 产品详情 |
| Edit |   |
|---|
| Product Name | PNGase F (CystatinA Tag), Recombinant, F. meningosepticum, aa41-354 |
|---|
| Description | Purity ~90% (SDS-PAGE). PNGase F, peptide Nglycosidase F from Flavobacterium meningosepticum, catalyzes the hydrolysis of asparaginelinked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins (1). Unlike glycosidases that hydrolyze glycosidic bonds, PNGase F is an amidase that cleaves the betaaspartylglucosamine bond between the innermost GlcNAc of Nglycans and asparagine residues of glycoproteins (2). The enzyme is highly active on various Nglycans except those with the innermost GlcNAc modified with alpha13linked core fucose, which is commonly found on plant Glycoproteins (3). Cleavage with PNGase F will convert the asparagine residue to an aspartic residue, allowing identification of the glycosylation sites by mass spectrometry (4). Recombinant PNGase F' is a fusion of human cystatin A to PNGase F. PNGase F' is a good alternative for PNGase F for deglycosylating proteins that have similar mass to PNGase F. |
|---|
| Size | 20ug |
|---|
| Concentration | n/a |
|---|
| Applications | n/a |
|---|
| Other Names | PNGF, Peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine Amidase F |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 145828 |
|---|
| Price | |
|---|
| Order / More Info | PNGase F (CystatinA Tag), Recombinant, F. meningosepticum, aa41-354 from UNITED STATES BIOLOGICAL |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|