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| Product Name | Alkaline Phosphatase, Calf Intestine, Conjugation grade (3000-6000u/mg) (AP) |
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| Description | Alkaline phosphatases (APs) are highly ubiquitous enzymes, present in all species from bacteria to man. In humans, APs are encoded by a multi-gene family composed of four loci; i.e., tissue- nonspecific AP, also called bone/liver/kidney AP, (Weiss et al, J. Biol. Chem., 264, 12002-12010, 1988), intestinal (Henthorn et. al., J.Biol. Chem., 263, 12020-12027, 1988). The sequence and complexity of the AP genes from other vertebrates and lower species are now being elucidated. The biological function of AP isozymes is still unknown. In vitro, the enzymes behave as phosphotransferases at neutral pH. The use of phosphate acceptor molecules (diethanolamine, tris, 2-amino-2-methyl-1- propanol) in the buffered substrate solutions increases the reaction rates and, thus, the sensitivity of assays based on AP determinations. ALP is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds |
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| Size | 1mg, 5mg, 10mg |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 208734 |
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| Order / More Info | Alkaline Phosphatase, Calf Intestine, Conjugation grade (3000-6000u/mg) (AP) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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