产品名称 EGFR (Epidermal Growth Factor Receptor) BioAssay™ ELISA Kit (Mouse) from UNITED STATES BIOLOGICAL
产品货号 369876
产品价格 现货询价,电话:010-67529703
产品规格 48 tests, 96 tests
产品品牌 UNITED STATES BIOLOGICAL
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Product NameEGFR (Epidermal Growth Factor Receptor) BioAssay™ ELISA Kit (Mouse)
DescriptionSample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody Sensitivity: <46.875pg/ml Range: 78.125-5000pg/ml Specificity: Mouse Test Principle: This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EGFR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EGFR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EGFR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of
Size48 tests, 96 tests
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Catalog #369876
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